| 质粒名称: | MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79248 |
| 存储实验室: | Martine Roussel |
|
载体骨架基本信息 |
|
| 载体名称: | MSCV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | p210 BCR-ABL |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | T1065S |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | IRES-GFP（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | pLXSN5' |
| 3' 测序引物 | pCDH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 79248 ; http://n2t.net/addgene:79248 ; RRID:Addgene_79248) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
- human p210BCR-ABL-IRES-GFP质粒图.png)
载体简介
载体序列
LOCUS MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP 13502 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION retroviral mammalian expression vector for human p210BCR-ABL and
GFP.
ACCESSION .
VERSION .
KEYWORDS MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13502)
TITLE Roussel lab plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 13502)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13502
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..517
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 581..922
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 989..1405
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1335..1357
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1373..1389
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(8608..8625)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8835..8854
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 9016..9735
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 9016..9732
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(9061..9082)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(9322..9341)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 9669..9690
/label=EGFP-C
/note="EGFP, forward primer"
LTR 9897..10411
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 10573..10589
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10597..10627)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10642..10663
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10780..10797)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10951..11539)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11031..11050)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11710..12570)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12333..12352
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12571..12675)
/gene="bla"
/label=AmpR promoter
primer_bind 12743..12761
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(12799..12821)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 12921..12940
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 13134..13156
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 13284..13303
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
1381 agccctcact ccttctctag gcgccggaat tgggatccca gtgtggtggt acgtaggaat
1441 tccccggcgc ccggggccgg gcctggcgag gccgccgcgc cgccgctgag acgggccccg
1501 cgcgcagccc ggcggcgcag gtaaggccgg ccgcgccatg gtggacccgg tgggcttcgc
1561 ggaggcgtgg aaggcgcagt tcccggactc agagcccccg cgcatggagc tgcgctcagt
1621 gggcgacatc gagcaggagc tggagcgctg caaggcctcc attcggcgcc tggagcagga
1681 ggtgaaccag gagcgcttcc gcatgatcta cctgcagacg ttgctggcca aggaaaagaa
1741 gagctatgac cggcagcgat ggggcttccg gcgcgcggcg caggcccccg acggcgcctc
1801 cgagccccga gcgtccgcgt cgcgcccgca gccagcgccc gccgacggag ccgacccgcc
1861 gcccgccgag gagcccgagg cccggcccga cggcgagggt tctccgggta aggccaggcc
1921 cgggaccgcc cgcaggcccg gggcagccgc gtcgggggaa cgggacgacc ggggaccccc
1981 cgccagcgtg gcggcgctca ggtccaactt cgagcggatc cgcaagggcc atggccagcc
2041 cggggcggac gccgagaagc ccttctacgt gaacgtcgag tttcaccacg agcgcggcct
2101 ggtgaaggtc aacgacaaag aggtgtcgga ccgcatcagc tccctgggca gccaggccat
2161 gcagatggag cgcaaaaagt cccagcacgg cgcgggctcg agcgtggggg atgcatccag
2221 gcccccttac cggggacgct cctcggagag cagctgcggc gtcgacggcg actacgagga
2281 cgccgagttg aacccccgct tcctgaagga caacctgatc gacgccaatg gcggtagcag
2341 gcccccttgg ccgcccctgg agtaccagcc ctaccagagc atctacgtcg ggggcatgat
2401 ggaaggggag ggcaagggcc cgctcctgcg cagccagagc acctctgagc aggagaagcg
2461 ccttacctgg ccccgcaggt cctactcccc ccggagtttt gaggattgcg gaggcggcta
2521 taccccggac tgcagctcca atgagaacct cacctccagc gaggaggact tctcctctgg
2581 ccagtccagc cgcgtgtccc caagccccac cacctaccgc atgttccggg acaaaagccg
2641 ctctccctcg cagaactcgc aacagtcctt cgacagcagc agtcccccca cgccgcagtg
2701 ccataagcgg caccggcact gcccggttgt cgtgtccgag gccaccatcg tgggcgtccg
2761 caagaccggg cagatctggc ccaacgatgg cgagggcgcc ttccatggag acgcagatgg
2821 ctcgttcgga acaccacctg gatacggctg cgctgcagac cgggcagagg agcagcgccg
2881 gcaccaagat gggctgccct acattgatga ctcgccctcc tcatcgcccc acctcagcag
2941 caagggcagg ggcagccggg atgcgctggt ctcgggagcc ctggagtcca ctaaagcgag
3001 tgagctggac ttggaaaagg gcttggagat gagaaaatgg gtcctgtcgg gaatcctggc
3061 tagcgaggag acttacctga gccacctgga ggcactgctg ctgcccatga agcctttgaa
3121 agccgctgcc accacctctc agccggtgct gacgagtcag cagatcgaga ccatcttctt
3181 caaagtgcct gagctctacg agatccacaa ggagttctat gatgggctct tcccccgcgt
3241 gcagcagtgg agccaccagc agcgggtggg cgacctcttc cagaagctgg ccagccagct
3301 gggtgtgtac cgggccttcg tggacaacta cggagttgcc atggaaatgg ctgagaagtg
3361 ctgtcaggcc aatgctcagt ttgcagaaat ctccgagaac ctgagagcca gaagcaacaa
3421 agatgccaag gatccaacga ccaagaactc tctggaaact ctgctctaca agcctgtgga
3481 ccgtgtgacg aggagcacgc tggtcctcca tgacttgctg aagcacactc ctgccagcca
3541 ccctgaccac cccttgctgc aggacgccct ccgcatctca cagaacttcc tgtccagcat
3601 caatgaggag atcacacccc gacggcagtc catgacggtg aagaagggag agcaccggca
3661 gctgctgaag gacagcttca tggtggagct ggtggagggg gcccgcaagc tgcgccacgt
3721 cttcctgttc accgacctgc ttctctgcac caagctcaag aagcagagcg gaggcaaaac
3781 gcagcagtat gactgcaaat ggtacattcc gctcacggat ctcagcttcc agatggtgga
3841 tgaactggag gcagtgccca acatccccct ggtgcccgat gaggagctgg acgctttgaa
3901 gatcaagatc tcccagatca agagtgacat ccagagagag aagagggcga acaagggcag
3961 caaggctacg gagaggctga agaagaagct gtcggagcag gagtcactgc tgctgcttat
4021 gtctcccagc atggccttca gggtgcacag ccgcaacggc aagagttaca cgttcctgat
4081 ctcctctgac tatgagcgtg cagagtggag ggagaacatc cgggagcagc agaagaagtg
4141 tttcagaagc ttctccctga catccgtgga gctgcagatg ctgaccaact cgtgtgtgaa
4201 actccagact gtccacagca ttccgctgac catcaataag gaagatgatg agtctccggg
4261 gctctatggg tttctgaatg tcatcgtcca ctcagccact ggatttaagc agagttcaaa
4321 agcccttcag cggccagtag catctgactt tgagcctcag ggtctgagtg aagccgctcg
4381 ttggaactcc aaggaaaacc ttctcgctgg acccagtgaa aatgacccca accttttcgt
4441 tgcactgtat gattttgtgg ccagtggaga taacactcta agcataacta aaggtgaaaa
4501 gctccgggtc ttaggctata atcacaatgg ggaatggtgt gaagcccaaa ccaaaaatgg
4561 ccaaggctgg gtcccaagca actacatcac gccagtcaac agtctggaga aacactcctg
4621 gtaccatggg cctgtgtccc gcaatgccgc tgagtatctg ctgagcagcg ggatcaatgg
4681 cagcttcttg gtgcgtgaga gtgagagcag tcctggccag aggtccatct cgctgagata
4741 cgaagggagg gtgtaccatt acaggatcaa cactgcttct gatggcaagc tctacgtctc
4801 ctccgagagc cgcttcaaca ccctggccga gttggttcat catcattcaa cggtggccga
4861 cgggctcatc accacgctcc attatccagc cccaaagcgc aacaagccca ctgtctatgg
4921 tgtgtccccc aactacgaca agtgggagat ggaacgcacg gacatcacca tgaagcacaa
4981 gctgggcggg ggccagtacg gggaggtgta cgagggcgtg tggaagaaat acagcctgac
5041 ggtggccgtg aagaccttga aggaggacac catggaggtg gaagagttct tgaaagaagc
5101 tgcagtcatg aaagagatca aacaccctaa cctggtgcag ctccttgggg tctgcacccg
5161 ggagcccccg ttctatatca tcactgagtt catgacctac gggaacctcc tggactacct
5221 gagggagtgc aaccggcagg aggtgaacgc cgtggtgctg ctgtacatgg ccactcagat
5281 ctcgtcagcc atggagtacc tggagaagaa aaacttcatc cacagagatc ttgctgcccg
5341 aaactgcctg gtaggggaga accacttggt gaaggtagct gattttggcc tgagcaggtt
5401 gatgacaggg gacacctaca cagcccatgc tggagccaag ttccccatca aatggactgc
5461 acccgagagc ctggcctaca acaagttctc catcaagtcc gacgtctggg catttggagt
5521 attgctttgg gaaattgcta cctatggcat gtccccttac ccgggaattg acctgtccca
5581 ggtgtatgag ctgctagaga aggactaccg catggagcgc ccagaaggct gcccagagaa
5641 ggtctatgaa ctcatgcgag catgttggca gtggaatccc tctgaccggc cctcctttgc
5701 tgaaatccac caagcctttg aaacaatgtt ccaggaatcc agtatctcag acgaagtgga
5761 aaaggagctg gggaaacaag gcgtccgtgg ggctgtgagt accttgctgc aggccccaga
5821 gctgcccacc aagacgagga cctccaggag agctgcagag cacagagaca ccactgacgt
5881 gcctgagatg cctcactcca agggccaggg agagagcgat cctctggacc atgagcctgc
5941 cgtgtctcca ttgctccctc gaaaagagcg aggtcccccg gagggcggcc tgaatgaaga
6001 tgagcgcctt ctccccaaag acaaaaagac caacttgttc agcgccttga tcaagaagaa
6061 gaagaagaca gccccaaccc ctcccaaacg cagcagctcc ttccgggaga tggacggcca
6121 gccggagcgc agaggggccg gcgaggaaga gggccgagac atcagcaacg gggcactggc
6181 tttcaccccc ttggacacag ctgacccagc caagtcccca aagcccagca atggggctgg
6241 ggtccccaat ggagccctcc gggagtccgg gggctcaggc ttccggtctc cccacctgtg
6301 gaagaagtcc agcacgctga ccagcagccg cctagccacc ggcgaggagg agggcggtgg
6361 cagctccagc aagcgcttcc tgcgctcttg ctccgcctcc tgcgttcccc atggggccaa
6421 ggacacggag tggaggtcag tcacgctgcc tcgggacttg cagtccacgg gaagacagtt
6481 tgactcgtcc acatttggag ggcacaaaag tgagaagccg gctctgcctc ggaagagggc
6541 aggggagaac aggtctgacc aggtgacccg aggcacagta acgcctcccc ccaggctggt
6601 gaaaaagaat gaggaagctg ctgatgaggt cttcaaagac atcatggagt ccagcccggg
6661 ctccagcccg cccaacctga ctccaaaacc cctccggcgg caggtcaccg tggcccctgc
6721 ctcgggcctc ccccacaagg aagaagctgg aaagggcagt gccttaggga cccctgctgc
6781 agctgagcca gtgaccccca ccagcaaagc aggctcaggt gcaccagggg gcaccagcaa
6841 gggccccgcc gaggagtcca gagtgaggag gcacaagcac tcctctgagt cgccagggag
6901 ggacaagggg aaattgtcca ggctcaaacc tgccccgccg cccccaccag cagcctctgc
6961 agggaaggct ggaggaaagc cctcgcagag cccgagccag gaggcggccg gggaggcagt
7021 cctgggcgca aagacaaaag ccacgagtct ggttgatgct gtgaacagtg acgctgccaa
7081 gcccagccag ccgggagagg gcctcaaaaa gcccgtgctc ccggccactc caaagccaca
7141 gtccgccaag ccgtcgggga cccccatcag cccagccccc gttccctcca cgttgccatc
7201 agcatcctcg gccctggcag gggaccagcc gtcttccacc gccttcatcc ctctcatatc
7261 aacccgagtg tctcttcgga aaacccgcca gcctccagag cggatcgcca gcggcgccat
7321 caccaagggc gtggtcctgg acagcaccga ggcgctgtgc ctcgccatct ctaggaactc
7381 cgagcagatg gccagccaca gcgcagtgct ggaggccggc aaaaacctct actcgttctg
7441 cgtgagctat gtggattcca tccagcaaat gaggaacaag tttgccttcc gagaggccat
7501 caacaaactg gagaataatc tccgggagct tcagatctgc ccggcgacag caggcagtgg
7561 tccagcggcc actcaggact tcagcaagct cctcagttcg gtgaaggaaa tcagtgacat
7621 agtgcagagg tagcagcagt caggggtcag gtgtcaggcc cgtcggagct gcctgcagca
7681 catgcgggct cgcccatacc cgtgacagtg gctgacaagg gactagtgag tcagcacctt
7741 ggcccaggag ctctgcgcca ggcagagctg agggccctgt ggagtccagc tctactacct
7801 acgtttgcac cgcctgccct cccgcacctt cctcctcccc gctccgtctc tgtcctcgaa
7861 ttttatctgt ggagttcctg ctccgtggac tgcagtcggc atgccaggac ccgccagccc
7921 cgctcccacc tagtgcccca gactgagctc tccaggccag gtgggaacgg ctgatgtgga
7981 ctgtcttttt catttttttc tctctggagc ccctcctccc ccggctgggc ctccttcttc
8041 cacttctcca agaatggaag cctgaactga ggccttgtgt gtcaggccct ctgcctgcac
8101 tccctggcct tgcccgtcgt gtgctgaaga catgtttcaa gaaccgcatt tcgggaaggg
8161 catgcacggg catgcacacg gctggtcact ctgccctctg ctgctgcccg gggtggggtg
8221 cactcgccat ttcctcacgt gcaggacagc tcttgatttg ggtggaaaac agggtgctaa
8281 agccaaccag cctttgggtc ctgggcaggt gggagctgaa aaggatcgag gcatggggca
8341 tgtcctttcc atctgtccac atccccagag cccagctctt gctctcttgt gacgtgcact
8401 gtgaatcctg gcaagaaagc tggaattcag atctcgagcg ggatcaattc cgcccccccc
8461 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
8521 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
8581 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
8641 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
8701 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
8761 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
8821 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
8881 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
8941 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
9001 aaacacgata ataccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
9061 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
9121 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
9181 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
9241 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
9301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
9361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
9421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
9481 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
9541 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
9601 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
9661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
9721 gagctgtaca agtaaagcgg ccgcgactct agagtcgacc tgcaggcatg caagcttcag
9781 gtagccggct aacgttaaca accggtacct ctagaactat agctagcatg cgcaaattta
9841 aagcgctgat atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
9901 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
9961 aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa
10021 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
10081 gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca
10141 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
10201 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
10261 actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac
10321 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
10381 gattgactac ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca
10441 acattctgag ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga
10501 atccacatac tccaatactc ctgaaatagt tcattatgga cagcgcagaa agagctgggg
10561 agaattgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
10621 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
10681 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
10741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
10801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
10861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
10921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
10981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
11041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
11101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
11161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
11221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
11281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
11341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
11401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
11461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
11521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
11581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
11641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
11701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
11761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
11821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
11881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
11941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
12001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
12061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
12121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
12181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
12241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
12301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
12361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
12421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
12481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
12541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
12601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
12661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
12721 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
12781 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
12841 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
12901 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
12961 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
13021 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
13081 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
13141 acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
13201 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
13261 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
13321 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt
13381 aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag
13441 cctatagagt acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg
13501 gg
//
